Prep HPLC Column, RP C4, Wide Pore, 5um, 300A. 21.2mm ID x 100mm Length. Cogent Brand. 1 EA. Special Order Item.
Additional Information:
Cogent RP C4™ Wide Pore is a reversed-phase HPLC stationary phase designed for the purification and isolation of proteins, peptides, enzymes, and other large hydrophobic or neutral compounds. Manufactured using a four-carbon butyl ligand bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore stationary phase provides enhanced accessibility for large biomolecules while maintaining the reduced hydrophobic retention characteristics of C4 chemistry. The wide pore design makes this phase particularly valuable for preparative bioseparations and biomolecule purification.
68504-P21-1003M is a preparative HPLC column packed with 5 µm RP C4™ Wide Pore particles featuring a 300 Å pore size in a 21.2 mm ID x 100 mm format. The large internal diameter provides substantial sample loading capacity for purification applications, while the 100 mm length offers an excellent balance between throughput and chromatographic resolution. The 5 µm particles provide lower operating backpressure than smaller particle sizes, making the column well suited for preparative HPLC systems. Typical flow rates for a 21.2 mm ID preparative column are approximately 15–30 mL/min depending on analyte characteristics, mobile phase composition, and system capabilities.
This column is particularly useful for the purification of proteins, peptides, enzymes, and other large biomolecules in pharmaceutical, biopharmaceutical, and research laboratories. The C4 bonded phase provides less hydrophobic retention than C8 and C18 phases, which can improve recovery and purification efficiency for larger analytes. Due to its size and flow requirements, a dedicated preparative HPLC system is required for operation.
Key Benefits
RP C4™ Wide Pore stationary phase
USP L26 equivalent chemistry
Butyl (C4) bonded phase
Fully double end capped
Ultra-high purity silica support
300 Å wide pore design
High sample loading capacity
Optimized for proteins and peptides
Lower hydrophobic retention than C8 phases
Suitable for preparative bioseparations
Typical Applications
Preparative HPLC
Protein purification
Peptide purification
Enzyme purification
Biomolecule isolation
Fraction collection
Biopharmaceutical purification
Process development
Method development
Laboratory-scale production
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP C4™ Wide Pore
USP Classification: L26
Chromatography Mode: Reversed Phase
Bonded Phase: Butyl (C4)
Endcapping: Fully Double End Capped
Particle Size: 5 µm
Pore Size: 300 Å
Column Dimensions: 21.2 mm ID x 100 mm
Length: 100 mm
Typical Flow Rate: 15–30 mL/min
Brand: Cogent
Special Order Item Information
Important Notes
Designed for proteins, peptides, enzymes, and other large biomolecules
300 Å wide pores improve accessibility for larger analytes
Provides less retention than C8 and C18 stationary phases
High loading capacity for preparative-scale purification
The 21.2 mm ID format requires a dedicated preparative HPLC system
Typical operating flow rates are approximately 15–30 mL/min
5 µm particles provide lower operating backpressure and robust performance
Fully double end capped to minimize secondary interactions
Excellent choice for preparative protein and peptide purification
This is a Special Order Item